Page 339 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka
infusion of cyperus rotundus and mimosa pudica was put into a sterile
test tube and then diluted with sterile distilled water without
concentrations 1:20, 1:30, 1:40, 1:50, 1:60, 1:70, 1:100, 1:110, 1:150,
1:200, 1:250. The solution was homogenized and transferred to
another sterile tube so that a final volume of 2ml was obtained
[21],[22],[23].
2.1.6. Phenol Coefficient Test
Prepared suspensions of test bacteria (S.aureus, S.epidermidis,
E.coli, P.aeruginosa, and S.typhi), tube racks, and sterile test tubes
containing NB media that have been labeled according to the dilution
and length of contact time (5, 10, and 15 minutes). 0.5 ml of the test
bacterial suspension was put into a tube containing the test treatment
(infusion of C.rotundus-M.pudica and control and 5% phenol) in
various dilution series starting from the tube with a dilution of 1:20 to
1:250, then homogenized. After 5 minutes, 1 ose is taken from each
test dilution series tube and put into each test tube containing the test
dilution series with a contact time of 5 minutes, then the used ose is
sterilized with Bunsen fire. After the second 5 minutes, it was carried
out in the same way for the test treatment with a contact time of 5
minutes (total contact time 10 minutes), and after the third 5 minutes
for the test treatment with a contact time of 5 minutes (total contact
time 15 minutes). The same phenol coefficient test steps were carried
out on each of the bacteria tested. All observation tubes were
incubated at 37°C for 24 hours, then observed for turbidity. The
presence of bacterial growth (+) is indicated by the medium becoming
cloudy, and the absence of bacterial growth (-) is indicated by the
medium remaining clear. Furthermore, the value of the phenol
coefficient is calculated with the following formula [21],[22],[23].

Lowest dilution of phenol that kills bacteria
{ Lowest dilution of antiseptic that kills bacteria }
= Highest dilution of phenol that kills bacteria
{ }
Highest dilution of antiseptic that kills bacteria

ISST 2022 – FST Universitas Terbuka, Indonesia 307
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0