Page 338 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
he 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka

at 90℃ and heated for 15 minutes, stirring occasionally [24]. The
results of the aqueous extract were filtered through filter paper while
hot and made several test concentrations. Then a single preparation
and a combination of infusion extracts were made according to the
concentration to be tested. The concentrations of the prepared
C.rotundus infusions were 50%, 75%, and 100% and 100% of
M.pudica infusions. The combination treatment was made with an
infusion mixture of each concentration of C.rotundus and M.pudica
infusion, with a ratio of 1:1.
2.1.3. Antibacterial Activity Test
Methods of testing antibacterial activity against bacterial cultures
using the dilution method (dilution) and standard culture. For each test
treatment of single and combined infusion, 1 ml of 70% alcohol control
was prepared and 0.1 ml of the test bacterial isolate suspension
(equivalent to 0.5 Mac Farland) was prepared, to be inserted into the
test medium aseptically. Infusion treatment and test control and
suspension of test bacteria were poured into a petri dish and a warm
NA liquid medium was added to cover the test solution. The crucible
was gently shaken to homogenize, then wrapped in aluminum foil and
incubated at 37°C for 24 hours. The results of the incubation will show
some colonies of test bacteria that grow on NA media, then the

number of bacterial colonies is calculated using a colony counter.
2.1.4. Preparation of phenol stock solution:
Phenol stock solution was prepared by dissolving 5 grams of phenol
crystals with 100 ml of sterile distilled water, so a phenol solution with
a concentration of 5% was obtained.
2.1.5. Dilution Phenol 5%
The 5% phenol solution was put into a sterile test tube that had been
labeled 1-13 as much as 2 ml, then distilled water was added to
it. After being added and then homogenized, then the phenol solution
mixture was transferred to the 13-36 tubes as much as 2 ml, then the
dilution of the phenol solution was 1:20, 1:30, 1:40, 1:50, 1:60, 1:70,
1: 80, 1:90, 1:100, 1:110, 1:150, 1:200, 1:250. Dilution infusion of
cyperus rotundus and mimosa pudica. a total of 1 ml of the combined

306 ISST 2022 – FST Universitas Terbuka, Indonesia
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0