Page 337 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka
of Staphylococcus aureus ATCC 25923, Escherichia coli
ATCC 25922, Salmonella typhi ATCC 19430, and Pseudomonas
aeruginosa ATCC 27853. The main ingredients used in this study
were the test plant Cyperus rotundus rhizome and Mimosa pudica
leaves as well as samples of pure isolates of bacterial colonies tested
from the Microbiology Laboratory of FK ULM. The chemicals used
were Nutrient Broth (NB) media, Brain Heart Infusion (BHI) media,
sterile distilled water, 5% phenol solution, and 0.5 Mc Farland
8
standard solution (equivalent to 1.5 X 10 CFU/mL).
2.1 Research procedure
2.1.1. Preparations of test Bacteria
Pure isolates of the test bacteria were prepared, then 1 ose was taken
and put into the BHI media to make a suspension of the test bacteria
cultures. Bacteria were incubated at 37°C for 24 hours. Then, sterile
distilled water was added to the bacterial suspension in BHI media so
that the turbidity was the same as the standard 0.5 Mc Farland
solution.
2.1.2. Making Simplicia and a Combination Infusion of Test
Plants
The simplicia material of C.rotundus rhizome and M.pudica leaves
were washed thoroughly with running water until separated from dirt,
then drained and avoided contamination. Furthermore, the simplicia
material is sliced into small pieces to expand the surface so that the
drying process is faster. The simplicia material is dried by aerating and
avoiding direct sunlight until the moisture content is <10% (easily
broken); dried in a blender to a powder. Simplicia powder is stored in
clean plastic and then placed in a room with low humidity, room
temperature (15°-30°C), smooth air circulation, and not exposed to
direct sunlight [24].
Simplicia made infusion preparations, namely by weighing the test
plant simplicia as much as 100 grams each and placing them in a
beaker glass container and adding 100 ml of distilled water, to make
a liquid preparation with a concentration of 100% w/v. Next, the glass
containing the herbal preparation was placed in an infusion pot bath

ISST 2022 – FST Universitas Terbuka, Indonesia 305
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0