Page 341 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka
The results of the combined infusion of C.rotundus and M.pudica
proved to show better antibacterial activity than a single preparation.
Furthermore, the effectiveness of combination infusions was tested by
comparison with antiseptic fluids, the effect was obtained as well as
antiseptics. This is because it produces a phenol coefficient value
equal to or more than the 5% phenol coefficient value. The results of
the phenol coefficient test are shown in Table. 2-4 and the coefficient
values are in Table. 5.
The phenol coefficient value of the combined infusion of C.rotundus
rhizomes and M.pudica leaves and 70% alcohol against
S.epidermidis, S.aureus, and E.coli was 1.10; for P.aeruginosa close
to the coefficient value of 5% phenol solution, which is 0.99, while for
S.typhi, it is 0.89. These results indicate the effectiveness of
combination infusions is relatively the same as the effectiveness of
antiseptics. The combination treatment of C.rotundus and M.pudica
infusion was proven to have better antibacterial activity than the single
preparations. The inhibition of bacterial colonization was affected by
an increase in the concentration of the infusion, so the number of
viable bacterial colonies decreased. This is the same as the
combination treatment of Piper battle L. and Ocimum sanctum L.
infusion on the number of test bacterial colonies.[22] The results of
this study are not much different from previous studies; gram-positive
bacteria were more sensitive to C.rotundus extract than gram-
negative bacteria.[13],[26] The effect of infusion on the number of
colonies of E.coli and P.aeruginosa is relatively the same, and the
effect is less on S.typhi.[22],[23]. It is like the previous research
reports; there was the antibacterial activity of M.pudica extract against
E.coli, P.aeruginosa, and S.aureus, but had no effect on S.typhi.[5]
Based on AgNPS biosynthesis, M.pudica leaf extract did not have
different antibacterial activity against E.coli and P.aeruginosa.[16]
Research using the diffusion method stated that the inhibition zone of
M.pudica showed the highest activity against E.coli and S.aureus,
while against P.aeruginosa the activity was moderate.[11]

ISST 2022 – FST Universitas Terbuka, Indonesia 309
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0