Page 340 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
he 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka

2.1.7. Data analysis
Research data to analyze antibacterial activity was tested using the
ANOVA test (one-way analysis of variance) and Duncan's post hoc
test, at a significance level of 95% (α = 0.05). [25] Data analysis of the
results of the phenol coefficient test to assess the effectiveness as an
antiseptic was carried out descriptively, namely by comparing the
coefficient value of the test treatment with the phenol coefficient value
of 5% phenol solution.
3 RESULTS
Infusion treatment of a single dosage form and a combination of
C.rotundus and M.pudica generally produced a better inhibitory effect
against gram-positive bacteria than gram-negative (Figure. 1). The
least number of test bacterial colonies was obtained from the 100%
(1:1) infusion combination treatment. The results of the ANOVA test
revealed that there were differences in the effect of all the treatments
tested. Duncan's test results (Table. 1.), showed that the average
number of colonies from the single infusion and combination
treatments of C.rotundus and M.pudica was significantly different. A
combination infusion of 100% (1:1) provides an effect equivalent to
70% alcohol treatment, which was tested on S. aureus and S.
epidermidis bacteria, except S.typhi.

150
of colonies 100
50
0
Average number (CFU/ml)

Treatments
S.epidermidis
S.aureus
E.coli

Figure 1. Infusion activity of Cyperus rotundus (Cr) rhizome and
Mimosa pudica (Mp) leaves against their age number of colonies of
Staphylococcus aureus, Staphylococcus epidermidis, Escherichia
coli, Pseudomonas aeruginosa, and Salmonella typhi.
308 ISST 2022 – FST Universitas Terbuka, Indonesia
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0