Page 336 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
he 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka

dosage form of herbal combinations (polyherbal) is more widely used
than single preparations, because of the synergistic effect of the
combination preparations which is more beneficial in increasing drug
activity [3]. An antiseptic can be said to have good effectiveness, it has
a phenol equivalent coefficient value of 5% based on the phenol
coefficient test. The phenol coefficient test is a test to determine the
effectiveness of an antiseptic preparation compared to 5% enol liquid
which has been proven to be effective as an antiseptic [23]. The
availability of C.rotundus and M.pudica plants which are quite
abundant in the community can be developed as alternative antiseptic
candidate preparations. This study aimed to analyze the antibacterial
activity of the infusion of Cyperus rotundus rhizome and Mimosa
pudica leaves in inhibiting the growth of several pathogenic bacteria
in humans. Based on the presence of various antibacterial compounds
in the rhizome of C.rotundus and M.pudica leaves, their activity in
inhibiting bacterial growth can be determined based on the
parameters of the number of tested bacterial colonies in vitro, while
the ability of the combined preparation of the two test plants to act as
an antiseptic is known through the coefficient test. phenol. The method
of infusion preparation was chosen in this study because the infusion
form has advantages in the availability of materials and ease of
manufacture.
2 METHODOLOGY
This true experimental research uses a posttest control group design.
Infusion treatment test rhizome combination of C.rotundus and
M.pudica leaves and control treatment using the dilution method. The
antibacterial activity of the infusion treatment and 70% alcohol control
was observed based on the number of bacterial colonies growing on
the test medium, while the activity of the treatment as an antiseptic
was calculated and analyzed based on the magnitude of the phenol
coefficient value. This research is a laboratory experiment conducted
at the Laboratory of Microbiology, Faculty of Medicine, the University
of Lambung Mangkurat in September 2021. The phenol coefficient
test method was carried out conventionally, using bacterial isolates

304 ISST 2022 – FST Universitas Terbuka, Indonesia
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0