Page 116 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka

Figure 5. Phage mechanism to infect bacteria within biofilm. (Collins,
2010)
Furthermore, the characteristics of phages that are considered to be
potential as an antibiofilm agents are phage can infect persister cells.
As mentioned above, antibiotics are not effective against metabolically
inactive bacteria or bacteria that are dormant or persister. On the other
hand, phages have the ability to infect persister bacteria[1]. A study by
Pearl et al.,[45] showed, persister bacteria are protected from phage
lysogenic mechanisms where the phage genetic material cannot
combine with the bacterial chromosome to form a prophage. However,
persister bacteria cannot avoid the lytic mechanism of the phage.
Quantitative analysis of gene expression showed that gene
expression related to the lytic mechanism would be suppressed during
bacterial dormancy. But when the bacteria are metabolically active
again and reproduce, the phage will re-express the gene and continue
the lysis mechanism [45].
Based on that, the bacteriophage can be alternative that has potential
to be antibiofilm agent for E. coli. Bumunang et al., [46] in his study
reported, the bacteriophage strain SA21RB could reduce the
formation of biofilm E. coli strain O154:H10 and O113:H21 after 24
hours. In this study, the assay was carried out using the microplate
phage virulence assay method where biofilm were formed on stainless

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International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0