Page 117 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka
steel plates then incubated for 24 hours, 48 hours, and 72 hours. After
incubation, the stainless steel plate was transferred to a petri dish
containing 5 mL of phage suspension with a concentration of 1013
PFU/mL for 3 hours, then the result showed that 1013 PFU/mL phage
suspension could reduce the biofilm by 2.5 and 2.1 log10 CFU/cm2 in
the biofilm that incubated for 24 hours.
Another study by Gonzales-Gomez et al., [47] also showed that
bacteriophage treatment PL-01, GB-02, and GB-03 could reduce E.
coli biofilm MGA-EC-27, MGA-EC25, and MGA-EC21. In this study,
screening was carried out on strains of E. coli bacteria that has ability
to formed biofilm. Then the best strains were obtained, MGA-EC-27,
MGA-EC25, and MGA-EC-21 which could form colonies in the biofilm
up to 8 log10 CFU/ cm2 with an incubation time of 24-120 hours.
Biofilm was formed on a stainless-steel plate and treated by adding
phage with a concentration of 108 or 109 PFU/mL for 1 hour. After
that, the population density in the biofilm was measured and the
results showed that there was a reduction of 0.95 log10 CFU/mL-6.70
log10 CFU/mL and the best concentration was at phage 109 PFU/mL.
Another study by Triana [48] also showed that the bacteriophage EC
RTH 04 showed 50-150% antibiofilm activity against E. coli biofilm.
Triana [48] tested phage activity against E. coli biofilm EC RTH4 using
the microplate flat bottom 96 wells assay method. This method begins
E. coli were cultured on a microplate and incubated thus biofilm can
be formed, then the biofilm is treated by adding a phage suspension
that has been diluted with serial dilution method until 108. The biofilm
activity was tested by 3 methods, prevention, inhibition, and
degradation. The result showed, the bacteriophage EC RTH 04 that
had isolated from toilet water in the Cimande area, Kab. Bogor has
activities to prevent, inhibit, and degrade biofilm where the highest
activity is degradation activity. In the degradation activity with
concentration of 104 reached 152.446%.

96 ISST 2022 – FST Universitas Terbuka, Indonesia
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0