Page 287 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka
d. Spermatozoa Morphology
Spermatozoa morphology was tested using eosin-nigrosine
staining. The procedure for making preparations is the same as
that for observing spermatoza viability. The preparations were
observed under a microscope with a magnification of 40 × 10.
Spermatozoa were observed in 10 fields of view or with a sperm
count of at least 200 cells. Spermatozoa morphology testing was
conducted to see the normality and abnormality of spermatozoa.
Spermatozoa were counted by dividing the number of abnormal
spermatozoa by the total number of spermatozoa counted
multiplied by 100%, with Equation (2):
Spermatozoa abnormality = (2
Σ abnormal spermatozoa )
× 100%
Σ total spermatozoa
2.7 Semen Processing
Semen/spermatozoa are mixed with diluent. Dilution can be calculated
by the following equation (3), where V = Semen volume (ml), M =
Spermatozoa motility (%), K = Spermatozoa concentration
(million/ml), IB dose = 25 million.
V × M × K (3)
Dilution volume (ml) = × 0.25
IB dose
2.8 Freezing and Spermatozoa Quality Testing after Freezing
Semen was packed in mini straws (0.25 ml) and then equilibrated at 5
°C for four hours. The freezing process was carried out using
Styrofoam measuring 38 × 27 × 27 by storing a 6 cm high straw rack
of liquid nitrogen (evaporated) for ten minutes. Frozen semen was
o
stored in liquid nitrogen (-196 C) for evaluation. The parameters of
frozen semen examination include motility, viability, the same as in
fresh semen. Furthermore, several attributes of the ecological
dimensions of the utilization of epididymal spermatozoa are discussed
using the DELPHI method, using controlled, iterative and feedback
information collection. To determine the leverage factors and the
sustainability status of the ecological dimension of the utilization of

ISST 2022 – FST Universitas Terbuka, Indonesia 257
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0