Page 281 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka
translocated to the middle area in the indentation of the flagellum [13].
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The concentration of spermatozoa in the corpus is 8-25 x 10 [22].
According to Gatti et al., [9], spermatozoa in this section already have
the ability to do zone bonding. Spermatozoa in this section have also
shown some fertility expressions [22]. The concentration of
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spermatozoa found in the cauda epididymis is 10-50 x 10 [22]. The
location of cytoplasmic droplets of spermatozoa in the cauda
epididymis has migrated more distally [13]. Spermatozoa in this
section have high disulfide cross-linking and have shown normal
motility [22]. The ability to fuse with the oolemma has been obtained
by spermatozoa in the cauda epididymis [9].
Diluent is a material that is added to semen to increase the volume of
semen, reduce the concentration of spermatozoa and maintain the
viability of spermatozoa for a certain time. A semen diluent solution
that has a more complete chemical composition will provide a good
function for the diluted semen, because the substrates in the diluent
are needed by spermatozoa to maintain their life [20]. The commonly
used semen diluent in Indonesia is Tris egg yolk which consists of Tris
Hydroxy methyl aminomethane, citric acid, fructose, egg yolk,
penicillin, streptomycin, and aquabidest. Tris together with citric acid
acts as a buffer to maintain pH changes due to the formation of lactic
acid from spermatozoa metabolism and plays a role in maintaining
osmolarity pressure and electrolyte balance [23]. Egg yolk contains
lipoproteins and lecithin which can maintain and protect the integrity
and lipoprotein envelope of spermatozoa cells [2]. Tris egg yolk diluent
was able to maintain semen quality in Pasundan cattle as well as
commercial diluents [2]. The content of fructose in the egg yolk Tris
diluent serves as an energy source so that spermatozoa can keep
moving. Fructose plays a role in producing energy in the form of ATP
which contains energy-rich organic phosphate and will be used for the
contraction of fibrils for the movement of spermatozoa. [6] added that
the viability of spermatozoa using egg yolk Tris diluent was higher in
various observation intervals, after dilution in Brebes Java cattle.

ISST 2022 – FST Universitas Terbuka, Indonesia 251
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0