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nd
               The 2  International Seminar of Science and Technology
               “Accelerating Sustainable innovation towards Society 5.0”
               ISST 2022 FST UT 2022
               Universitas Terbuka
               the  muscle  was  chopped  and  finally  ground  with  a  blender  for
               homogenous mixture [3].
               Biochemical  analysis:  Proximate  composition:  AOAC  method  was
               followed  for  proximate  composition  of  the  fresh  and  frozen  prawn.
               Moisture: The loss of moisture was calculated with the Equation (1).
                                              ℎ                               −        ℎ                               
                Moisture content (%) =                                   (1)
                                                      ℎ                               
                (100)
               Detection of unwanted materials: Collected tissues were weighed by
               electronic balance and 5 ml of diacid mixture (5 ml conc. HNO3: 3 ml
               60%  HClO4)  were  added  to  each  sample.  The  content  mixed  for
               overnight. Samples were then digested, initially at 80°C and later at
               150°C  for  2  hours.  The  completion  of  digestion  was  indicated  by
               almost  colorless  material.  The  samples  were  analyzed  using  the
               Atomic Absorption Spectrophotometer method of Clesceri et al. The
               Equation (2) calculated the concentration of heavy metal.
                Metal concentration  =                                 (2)
                                                                                                                (    )

                                    ℎ                                         
               3  RESULTS
               Change in physical characteristics during fresh and frozen storage:
               Table.  1  shows  the  change  in  organoleptic  qualities  on  prawn
               (Macrobrachiumrosenbergii)  during  frozen  storage  at  -20°C.  It  was
               organoleptically  in  acceptable  condition  for  8  weeks.  During
               observation up to 4th weeks it was in excellent condition with natural
               odour and flavour. The characteristics of bright appearance, soft and
               firm texture also present. During 8th week of observation there was
               little deterioration apart from some loss of natural odour and still the
               off flavour was not introduced. During 12th weeks of observation there
               was loss of bright appearance, loss of natural odour, the loss of natural
               flavour  was  observed,  and  the  experimental  agent  was  in  rejected
               condition [4]. The quality of prawn was acceptable up to 4 hours in all
               case according to organoleptic assessment, but this score gradually
               decreases.




               ISST 2022 – FST Universitas Terbuka, Indonesia            183
               International Seminar of Science and Technology “Accelerating Sustainable
               Towards Society 5.0
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