Page 376 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka
spectrometry (AAS) method [10]. Each of the samples were divided
into two parts, 0.5 mL for positive control and 0.5 mL for the sample.
Added 0.25 mL of 1 mg / l standard solution to the sample to make
spiked or positive control. Spiked is evaporated on a hot plate at 100
ºC until dry. The sample and spiked are put into an ashing furnace and
cover half of the surface. The temperature of the ashing furnace is
gradually increased by 100 ºC every 30 minutes until it reaches 450
ºC and is maintained for 18 hours.
The sample and spiked were removed from the ashing furnace and
cooled to room temperature. After chilling, 1 mL of HNO3 65% was
added, shaken carefully so that all the ash dissolved in the acid and
then evaporated on a hot plate at 100 ºC until dry. After drying the
sample and spiked are put back into the ashing furnace. The
temperature is gradually increased by 100 ºC every 30 minutes until it
reaches 450 ºC and is maintained for 3 hours. After the ash is
completely white, the sample and spiked are cooled at room
temperature. 5 mL of 6 M HCl was added to each sample and the
spiked was carefully shaken so that all the ash dissolved in the acid.
Evaporated on a hot plate at 100 ºC until dry. 10 mL of 0.1 M HNO3
was added and cooled at room temperature for 1 hour, the solution
was transferred to a 50 mL polypropylene measuring flask and added
to the matrix modifier solution, adjusting it to the boundary mark using
0.1 M HNO3. The working standard lead solution was prepared
respectively. A minimum of five concentration points each. Standard
working, sample, and spiked solutions were read on a graphite fumace
atomic absorption spectrophotometer at a wavelength of 288.3 nm for
lead heavy metal.
3 RESULTS
From the 270 cattle blood samples examined, amount 20 samples
were found positive for lead heavy metal contamination. The average
levels of the lead heavy metal in cattle blood (0.109 ± 0.080 ppm) did
not exceed the maximum threshold level for consumption, namely
1.00 ppm (BSN, 2009). Meanwhile, the lead content in soil and

ISST 2022 – FST Universitas Terbuka, Indonesia 339
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0