Page 354 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka
(transmembrane proteins) on the outer membrane of the bacterial cell
wall and form strong polymeric bonds that damage the porins.[38]
Damage to the porin, which is the entry and exit point for compounds
needed by the bacterial cell, will reduce the permeability of the
bacterial cell wall so that the bacterial cell lacks nutrients so that the
growth of the bacteria is inhibited or dies.[35],[39] Tannins are
polyphenolic compounds that dissolve in water. Tannins are
derivatives of 3,4,5-trihydroxy benzoic acid. Tannins can bind to
proteins, forming complex compounds with proteins through hydrogen
bonds. Hydrogen bonding between tannins and proteins is affected by
temperature, pH, solution composition, and the tannin: protein
ratio.[40]
The results of this study were relatively the same as previous studies,
namely the combined infusion test results of Averrhoa bilimbi and
Cananga odorata [22] and the combined infusion of Piper battle L. and
Ocimum sanctum L. [23]; both tests use the same type of test bacteria.
This study can prove the effectiveness of the infusion combination as
an antiseptic based on the parameters of the number of bacterial
colonies and the value of the phenol coefficient. So that the
combination infusion of C.rotundus and M.pudica has the potential to
be developed as a natural antiseptic candidate. The next stage can be
tested organoleptic and its effectiveness in vivo.

4 CONCLUSIONS
The antibacterial activity of C.rotundus rhizome infusion and M.pudica
leaves as natural antiseptic candidates was proven in this study, with
parameters of the number of bacterial colonies and their phenol
coefficient values against several laboratory standard bacteria. In
conclusion, the combination of C.rotundus and M.pudica infusion had
good antibacterial activity in inhibiting test bacterial colonization and
had a phenol coefficient value equivalent to that of the antiseptic
control.

ISST 2022 – FST Universitas Terbuka, Indonesia 317
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0