Page 352 - Proceeding The 2nd International Seminar of Science and Technology : Accelerating Sustainable Innovation Towards Society 5.0

nd
The 2 International Seminar of Science and Technology
“Accelerating Sustainable innovation towards Society 5.0”
ISST 2022 FST UT 2022
Universitas Terbuka
The combination of C.rotundus 100% and 100% M.pudica infusion
had an effect that was not significantly different from 70% alcohol on
all bacteria, except for S.typhi which had a different effect at
concentrations <100%. This proves the achievement of a synergistic
effect, which strengthens each other at the combined concentrations.
The results of this study are in accordance with the results of the
combined infusion test of Stanochlaena palustris and Sauropus
androgynous leaves against E.coli, S.aureus, and C.albicans bacteria;
the inhibitory effect was greater than that of the single preparation and
the effect was equivalent to that of the positive control. [27] The
synergistic effect of several herbs can provide more beneficial
effects.[3] Significant inhibitory effect of polyherbal plant extracts on
S.aureus, P.aeruginosa, B.subtillis, and E.coli; so that polyherbal
formulations can be used in commercial scale hand washing herbal

preparations.[8]
The same results were obtained for the phenol coefficient parameter.
Comparison of the results of the phenol coefficient test between the
combination infusion preparations with antiseptic control and 5%
phenol proved the effectiveness of this combination infusion as a good
antiseptic, i.e. the equivalent value of the phenol coefficient is obtained
≥1.[22],[23] Its effectiveness as an antiseptic preparation against
gram-positive bacteria is stronger than gram-negative bacteria.The
infusion preparations from the two test plants contained various
bioactive compounds to inhibit bacterial growth/colonization. The
antibacterial effect is influenced by the level, type, and polarity of the
secondary compound content. In the infusion method, most of the
polar compounds and some semi-polar compounds are dissolved in
aqueous solvents. Compounds in C.rotundus such as alkaloids,
cineol, pinene, cyproterone, retinol, eplerenone, tannins, cyperols,
and flavonoids;[7] as well as glycosides, anthraquinones, saponins,
steroids, and triterpenoids, have antibacterial properties.13,26
Secondary compounds in M.pudica that are antibacterial include
tannins, saponins, steroids, flavonoids; glycosides, non-protein amino
acid leucine (mimosine), alkaloids, steroids, tannins, triterpenes,
flavonoids, glycosides, quinine, phenols, saponins, coumarins, c-
glycosyl-flavones; [28],[29] also tyrosine, and mimosinamine.[5]

ISST 2022 – FST Universitas Terbuka, Indonesia 315
International Seminar of Science and Technology “Accelerating Sustainable
Towards Society 5.0